Clokie Martha R.J., Kropinski A. (eds.) Bacteriophages: Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions

Humana Press, 2009. — 313 p. — (Methods in Molecular Biology 501) — ISBN: 978-1-58829-682-5.Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 1: Isolation, Characterization, and Interactions examines a multitude of topics, including the isolation of phages, morphological and molecular characterization, and interaction with bacteria. Written in the highly successful Methods in Molecular Biology series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls.Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.Methods for the Isolation of Viruses from Environmental Samples
Bacteriophage Enrichment from Water and Soil
Isolation of Phage via Induction of Lysogens
Isolation of Cyanophages from Aquatic Environments
Isolation of Viruses from High Temperature Environments
Isolation of Novel Large and Aggregating Bacteriophages
Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay
Enumeration of Bacteriophages by the Direct Plating Plaque Assay
Enumeration of Bacteriophages Using the Small Drop Plaque Assay System
Determination of Virus Abundance by Epifluorescence Microscopy
Enumeration of Bacteriophages Using Flow Cytometry
Basic Phage Electron Microscopy
Phage Classification and Characterization
Phage Host Range and Efficiency of Plating
Measurement of the Rate of Attachment of Bacteriophage to Cells
Measurement of the Bacteriophage Inactivation Kinetics with Purified Receptors
Bacteriophage Plaques: Theory and Analysis
Practical Methods for Determining Phage Growth Parameters
Phage Production and Maintenance of Stocks, Including Expected Stock Lifetimes
Construction of Phage Mutants
Modifying Bacteriophage $\lambda$ with Recombineering
Identification and Isolation of Lysogens with Induced Prophage
Generalized Transduction
Preparation and Characterization of Anti-phage Serum
Generalized Transduction by Lytic Bacteriophages