Granato Paul A., Morton V., Morello J.A. (eds.) Laboratory Manual and Workbook in Microbiology: Applications to Patient Care

12th edition. — McGraw-Hill Professional Publishing, 2019. — 337 p. — ISBN: 9781260002188This lab manualemphasizes the basic principles of diagnostic microbiology for studentspreparing to enter the allied health field. Students are led through a seriesof exercises that allow them to learn basic microbiology techniques andpractice safety in the laboratory and hospital environment. It is primarily orientedtoward meeting the interests and needs of those who will be directly involvedin patient care and wish to learn how microbiological principles should beapplied in the practice of their professions.The authors articulatethe purposes and function of the clinical microbiology laboratory in thediagnosis of infectious diseases. Exercises illustrate the nature of laboratoryprocedures used for isolation and identification of infectious agents as wellas the principles of asepsis, disinfection and sterilization. The role of thehealth professional is highlighted in regard to appropriate collection ofclinical specimens and application of aseptic and disinfectant techniques asthey relate to patient care.Basic Techniques of Microbiology
The Microscope
Handling and Examining Cultures
Simple Stains, Hanging Drop, and Wet-Mount Preparations
Gram Stain
Acid-Fast Stain
Culture Media
Streaking Technique to Obtain Pure Cultures
Subculture Techniques
Destruction of Microorganisms
Moist and Dry Heat
The Autoclave
Disinfectants
Antimicrobial Agent Susceptibility Testing and Resistance
Diagnostic Microbiology in Action
Primary Media for Isolation of Microorganisms
Some Metabolic Activities of Bacteria
Activities of Bacterial Enzymes
Antigen Immunoassays for Detection and Identification of Microogranisms
Principles of Nucleic Acid Assays for Multiplex Syndrome Panel Testing for the Diagnosis of Infectious Disease
Principles and Applications of MALDI-TOF Mass Spectrometry for the Rapid Identification of Bacteria and Fungi
Streptococci
Streptococci, Pneumococci, and Enterococci
Haemophilus, Corynebacteria, and Bordetella
Clinical Specimens from the Respiratory Tract
The Enterobacteriaceae (Enteric Bacilli) and Other Clinically Important Gram-Negative Bacilli
Clinical Specimens from the Intestinal Tract
Urine Culture Techniques
Neisseria and Spirochetes
Clinical Specimens from Blood Case Study
Microbial Pathogens Requiring Special Laboratory Techniques
Serodiagnosis of Infectious Disease
Principles and Practices of Infection Prevention
Anaerobic Bacteria
Mycobacteria
Mycoplasmas, Rickettsiae, Chlamydiae, Viruses, and Prions
Fungi:Yeasts and Molds
Protozoa and Animal Parasites
Serodiagnosis of Infectious Disease
Principles and Practices of Infection Prevention