Loyola-Vargas V.M., Ochoa-Alejo N. Plant Cell Culture Protocols

3rd ed. — Humana Press, 2012 — 430 p. — (Methods in Molecular Biology 877) — ISBN: 978-1-61779-818-4.Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. “Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.”Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.An Introduction to Plant Cell Culture: The Future Ahead
History of Plant Tissue Culture
Callus, Suspension Culture, and Hairy Roots. Induction, Maintenance and Characterization
Growth Measurements: Estimation of Cell Division and Cell Expansion
Measurement of Cell Viability
Pathogen and Biological Contamination Management in Plant Tissue Culture: Phytopathogens, Vitro Pathogens, and Vitro Pests
Cryopreservation of Embryogenic Cell Suspensions by Encapsulation–Vitrification and Encapsulation–Dehydration
The Study of In Vitro Development in Plants: General Approaches and Photography
Use of Statistics in Plant Biotechnology
Tissue Culture Methods for the Clonal Propagation and Genetic Improvement of Spanish Red Cedar (Cedrela odorata)
Micropropagation of Banana
Liquid In Vitro Culture for the Propagation of Arundo donax
Production of Haploids and Doubled Haploids in Maize
Maize Somatic Embryogenesis: Recent Features to Improve Plant Regeneration
Improved Shoot Regeneration from Root Explants Using an Abscisic Acid-Containing Medium
Cryopreservation of Shoot Tips and Meristems: An Overview of Contemporary Methodologies
Anther Culture of Chili Pepper (Capsicum spp.)
Production of Interspecific Hybrids in Ornamental Plants
Plant Tissue Culture of Fast-Growing Trees for Phytoremediation Research
Removing Heavy Metals by In Vitro Cultures
Establishment of a Sanguinarine-Producing Cell Suspension Culture of Argemone mexicana L (Papaveraceae): Induction of Alkaloid Accumulation
Epigenetics, the Role of DNA Methylation in Tree Development
The Potential Roles of microRNAs in Molecular Breeding
Determination of Histone Methylation in Mono- and Dicotyledonous Plants
Basic Procedures for Epigenetic Analysis in Plant Cell and Tissue Culture