Hatada I. Genome Editing in Animals

Humana Press, 2017. — 258 p. — (Methods in Molecular Biology 1630). — ISBN: 978-1-4939-7128-2.This volume details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Genome Editing in Animals: Methods and Protocols aims to ensure successful results in the further study of this vital field.Construction and Evaluation of Zinc Finger Nucleases
Current Overview of TALEN Construction Systems
CRISPR/Cas9
Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse
Genome Editing of Mouse by Cytoplasmic Injection
Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing SgRNA/Cas9 Expressing Plasmids
Genome Editing in Mouse and Rat by Electroporation
Generation of Knock-in Mouse by Genome Editing
Genome Editing of Rat
Gene Targeting in Rabbits: Single-Step Generation of Knock-out Rabbits by Microinjection of CRISPR/Cas9 Plasmids
Genome Editing of Pig
Genome Editing of Monkey
Genome Editing Mediated by Primordial Germ Cell in Chicken
CRISPR/Cas9-Mediated Targeted Knockin of Exogenous Reporter Genes in Zebrafish
Genome Editing of Medaka
A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders Using the CRISPR-Cas System
Genome Editing of Silkworms
Genome Editing in the Cricket, Gryllus bimaculatus
Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease
Genome Editing of C. elegans