Bhalla P.L., Singh M. Wheat Biotechnology

Humana Press, 2017. — 309 p. — (Methods in Molecular Biology 1679). — ISBN: 978-1-4939-7337-8.This detailed volume provides background on recent new technology developments highlighting the potential of the genomic era in wheat breeding with invaluable instruction on the methodology, which is complemented by overview chapters on the status of new technology application in major wheat production countries. The topics, addressed by internationally renowned scientists active in the field, cover methods underpinning the latest developments in the field of wheat biotechnology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Wheat Biotechnology: Methods and Protocols serves as a vital resource for scientists working to breed future high-yielding wheat varieties to sustain a growing population in an increasingly unpredictable world.Enabling Molecular Technologies for Trait Improvement in Wheat
What Will Be the Benefits of Biotech Wheat for European Agriculture?
Overview of the Wheat Genetic Transformation and Breeding Status in China
Wheat Improvement in India: Present and Future
Overview of Methods for Assessing Salinity and Drought Tolerance of Transgenic Wheat Lines
Allergenicity Assessment of Transgenic Wheat Lines In Silico
Agribusiness Perspectives on Transgenic Wheat
Agrobacterium-Mediated Transformation of Wheat Using Immature Embryos
Biolistic Transformation of Wheat
Wheat Genetic Transformation Using Mature Embryos as Explants
Targeted Mutagenesis in Hexaploid Bread Wheat Using the TALEN and CRISPR/Cas Systems
Design and Assembly of CRISPR/Cas9 Reagents for Gene Knockout, Targeted Insertion, and Replacement in Wheat
Doubled Haploid Transgenic Wheat Lines by Microspore Transformation
Doubled Haploid Laboratory Protocol for Wheat Using Wheat–Maize Wide Hybridization
Real-Time PCR for the Detection of Precise Transgene Copy Number in Wheat
Endogenous Reference Genes and Their Quantitative Real-Time PCR Assays for Genetically Modified Bread Wheat (Triticum aestivum L.) Detection
Phenotypic Characterization of Transgenic Wheat Lines Against Fungal Pathogens Puccinia triticina and Fusarium graminearum
Databases for Wheat Genomics and Crop Improvement
High-Density SNP Genotyping Array for Hexaploid Wheat and Its