Hacker, David L. Recombinant Protein Expression in Mammalian Cells

Humana Press, 2018. — 311 p. — (Methods in Molecular Biology 1850). — ISBN: 978-1-4939-8730-6 (eBook).About this book
This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.Transient Gene Expression in Suspension HEK293-EBNA1 Cells Transient Expression of Recombinant
Membrane-eGFP Fusion Proteins inHEK293Cells
PEI-Mediated Transient Gene Expression in CHO Cells
Stable Expression by Lentiviral Transduction of Cells
Inducible Protein Production in 293 Cells Using the piggyBac Transposon System
Recombinant CHO Cell Pool Generation Using piggyBac Transposon System
Protein Expression via Transient Transfection of Mammalian Cells in a WAVEBioreactor
Continuous and Integrated Expression and Purification of Recombinant Antibodies
High Throughput Transfection of HEK293 Cells for Transient Protein Production
Microfluidic Transfection for High-Throughput Mammalian Protein Expression
Targeting miRNAs with CRISPR/Cas9 to Improve Recombinant Protein Production of CHO Cells
Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV)