Rønning S.B. (ED.) Myogenesis: Methods and Protocols

Springer, 2019. — 353 p. — (Methods in Molecular Biology 1889). — ISBN: 1493988964.This detailed volume collects many of the common experimental approaches used to study myogenesis. It covers subjects ranging from isolation and purification protocols, manipulation of muscle cells, transcriptomics and proteomics, metabolism and exercise, and tissue engineering. Presented methods involve different species, including human, bovine, Atlantic salmon, rats, mice, larval zebrafish, and Drosophila melanogaster. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS)
Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells
Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD
Chromatin Immunoprecipitation in Skeletal Myoblasts
Exercising Bioengineered Skeletal Muscle In Vitro: Biopsy to Bioreactor
Isolation and Purification of Satellite Cells from Young Rats by Percoll Density Gradient Centrifugation
Lentivirus-Mediated RNAi in Skeletal Myogenesis
Adipogenesis from Bovine Precursors
Transcriptomic Profiling During Myogenesis
Coculture Method to Obtain Endothelial Networks Within Human Tissue-Engineered Skeletal Muscle
Interaction Between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System
LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue
A siRNA Mediated Screen During C2C12 Myogenesis
Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle
Stem/Progenitor Cells Following Skeletal Muscle Injury
Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis
Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis
Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers
Myoblast Phosphoproteomics as a Tool to Investigate Global Signaling Events During Myogenesis
Preparation and Culturing of Atlantic Salmon Muscle Cells for In Vitro Studies
RNA Interference Screening for Genes Regulating Drosophila Muscle Morphogenesis