Dutta D. Microfluidic Electrophoresis: Methods and Protocols

Springer, 2019. — 259 p. — (Methods in Molecular Biology 1906). — ISBN: 1493989634.This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Fabrication of Glass Microfluidic Devices
Soft Lithography, Molding, and Micromachining Techniques for Polymer Micro Devices
Sample Injection Techniques
Sample Preconcentration Protocols in Microfluidic Electrophoresis
Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection
Micellar Electrokinetic Chromatography
Microchip Isotachophoresis: Analysis of Pharmaceuticals
Microfluidic Free-Flow Isoelectric Focusing with Real-Time pI Determination
Nanochannel Gradient Separations
Paper-Based Electrophoresis Microchip as a Powerful Tool for Bioanalytical Applications
Band Broadening Theories in Capillary Electrophoresis
Estimating Stream Broadening in Free-Flow Electrophoretic Systems Based on the Method-of-Moments Formulation
Microchip Electrophoresis Tools for the Analysis of Small Molecules
Integrated Microfluidic System for Rapid DNA Fingerprint Analysis: A Miniaturized Integrated DNA Analysis System (MiDAS)—Swab Sample-In to DNA Profile-Out
Achieving Stable Electrospray Ionization Mass Spectrometry Detection from Microfluidic Chips
Microchip-Based Electrophoretic Separations with a Pressure-Driven Backflow