Santos N.C, Carvalho F.A. (Eds.) Atomic Force Microscopy: Methods and Protocols

Springer, 2019. — 369 p. — (Methods in Molecular Biology 1886). — ISBN: 149398893X.This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image artifacts, process and quantitatively analyze AFM images, lipid bilayers, image DNA-protein complexes, AFM cell topography, single-molecule force spectroscopy, single-molecule dynamic force spectroscopy, fluorescence methodologies, molecular recognition force spectroscopy, biomechanical characterization, AFM-based biosensor setup, and detail how to implement such an in vitro system, which can monitor cardiac electrophysiology, intracellular calcium dynamics, and single cell mechanics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Atomic Force Microscopy: Methods and Protocols is useful for researchers at different stages, from newcomers to experienced users, interested in new AFM applications.Parti Imaging
Artifacts and Practical Issues in Atomic Force Microscopy
Quantitative Analysis of Structure and Dynamics in AFM Images of Lipid Membranes
Imaging Artificial Membranes Using High-Speed Atomic Force Microscopy
Investigating the Nanodomain Organization of Rhodopsin in Native Membranes by Atomic Force Microscopy
Analysis of DNA–Protein Complexes by Atomic Force Microscopy
Imaging: The Case of TRF2–Telomeric DNA Wrapping
Cell Topography and Its Quantitative Imaging by AFM
Single-Molecule Force Spectroscopy
Functionalization of AFM Tips and Supports for Molecular
Recognition Force Spectroscopy and Recognition Imaging
Ligand-Receptor Binding on Cell Membrane: Dynamic
Force Spectroscopy Applications
Single-Molecule Force Spectroscopy: Experiments, Analysis, and Simulations
AFM to Study Pore-Forming Proteins
Imaging and Manipulation of Extracellular Traps by Atomic Force Microscopy
Studies Of Bacteria And Virus in AFM
Investigation of Bacterial Curli Production and Adhesion Using AFM
Antimicrobial Peptides: Effect on Bacterial Cells
AFM Nanoindentation Experiments on Protein Shells: A Protocol
Structural and Mechanical Characterization of Viruses with AFM
AFM Elasticity Studies
Quantification of the Elastic Properties of Soft and Sticky
Materials Using AFM
Measuring the Elastic Properties of Living Cells
Measuring Elastic Properties of Single Cancer Cells by AFM
AFM Applications in Nanomedicine
Molecular Recognition Force Spectroscopy for Probing Cell Targeted Nanoparticles In Vitro
Biomechanical Characterization of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy
Simultaneous AFM Investigation of the Single Cardiomyocyte Electro-Chemo-Mechanics During Excitation-Contraction Coupling