Matthew McKenzie. Mitochondrial DNA. Methods and Protocols

New York: Humana Press, 2016. — 234 p.
Series: Methods in Molecular Biology.
ISBN: 978-1-4939-3039-5This third edition of Mitochondrial DNA : Methods and Protocols describes these new methodologies for mitochondrial DNA analysis and, combined with well-established protocols that are considered the gold standard in the fi eld, comprises a compendium of detailed methods for the study of mitochondrial DNA biology. The fi rst part describes protocols for detecting mutations in mitochondrial DNA by next-generation massive parallel sequencing as well as techniques to assess mitochondrial DNA damage. The second part describes the visualization of mitochondrial DNA in situ and the detection of mitochondrial DNA nucleoids within the mitochondria. The third part describes methods for analyzing mitochondrial DNA replication, mitochondrial DNA-encoded protein translation, and mitochondrial DNA copy number. The fourth part describes the latest technology for modifying the mitochondrial genome, while the fi fth part describes how to purify proteins involved in the replication and transcription of mitochondrial DNA.