Moya A., Pérez Brocal V. (eds.) The Human Virome. Methods and Protocols

Humana Press, 2018. — xi + 274 p. — ISBN: 978-1-4939-8681-1.The aim of this book is to compile the most comprehensive and up-to-date collection of cutting-edge protocols used in wet and dry lab to investigate the viral communities harbored within and on the human body. A practical and experience-based book, it includes methods for collection, isolation, identification, and computational/statistical analysis of the human virome. This volume is arranged by body niches to cover those methodological issues inherent to the human tissues and organs, facilitating the researchers their daily work from beginning to end in the field of the research on the human virome.General Experimental Methods
Flow Cytometry and Direct Sequencing of Viruses
Tissue-Based Universal Virus Detection (TUViD-VM) Protocol for Viral Metagenomics
Protocol for Generating Infectious RNA Viromes from Complex Biological Samples
Phage on Tap: A Quick and Efficient Protocol for the Preparation of Bacteriophage Laboratory StocksGastrointestinal Tract
Extraction and Purification of Viruses from Fecal Samples for Metagenome and Morphology Analyses
Virome Sequencing of Stool Samples
NetoVIR: Modular Approach to Customize Sample Preparation Procedures for Viral Metagenomics
Viral Genome Isolation from Human Faeces for Succession Assessment of the Human Gut ViromeRespiratory Tract
Introduction to Techniques and Methodologies for Characterizing the Human Respiratory Virome
Targeted Sequencing of Respiratory Viruses in Clinical Specimens for Pathogen Identification and Genome-Wide AnalysisOral Cavity
Methods for Enrichment and Sequencing of Oral Viral Assemblages: Saliva, Oral Mucosa, and Dental Plaque ViromesBlood–Plasma
A Method for Isolation of the Virome from Plasma Samples
Viral Concentration and Amplification from Human Serum Samples Prior to Application of Next-Generation Sequencing AnalysisUrinary Tract
Identification and Quantification of DNA Viral Populations in Human Urine Using Next-Generation Sequencing ApproachesBioinformatics
Diversity Analysis in Viral Metagenomes
Construction of a Comprehensive Database from the Existing Viral Sequences Available from the International Nucleotide Sequence Database Collaboration
Robust Analysis of Time Series in Virome Metagenomics
Bioinformatics Assembling and Assessment of Novel Coxsackievirus B1 Genome