Jeltsch Albert, Rots Marianne G. (eds.) Epigenome Editing: Methods and Protocols

Humana Press, 2018. — 476 p. — (Methods in Molecular Biology 1767). — ISBN: 978-1-4939-7774-1This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Editing the Epigenome: Overview, Open Questions, and Directions of Future Development
Zinc Fingers, TALEs, and CRISPR Systems: A Comparison of Tools for Epigenome Editing
Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities
Generation of TALE-Based Designer Epigenome Modifiers
Neuroepigenetic Editing
Allele-Specific Epigenome Editing
Key to Delivery: The (Epi-)genome Editing Vector Toolbox
CRISPR/dCas9 Switch Systems for Temporal Transcriptional Control
Delivery of Designer Epigenome Modifiers into Primary Human T Cells
Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniques
Stable Expression of Epigenome Editors via Viral Delivery and Genomic Integration
Purified Protein Delivery to Activate an Epigenetically Silenced Allele in Mouse Brain
Non-viral Methodology for Efficient Co-transfection
Chromatin Immunoprecipitation in Human and Yeast Cells
Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis
Generation of Whole Genome Bisulfite Sequencing Libraries for Comprehensive DNA Methylome Analysis
Approaches for the Analysis and Interpretation of Whole Genome Bisulfite Sequencing Data
Whole-Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution
Locus-Specific DNA Methylation Analysis by Targeted Deep Bisulfite Sequencing
DNA Methylation Analysis by Bisulfite Conversion Coupled to Double Multiplexed Amplicon-Based Next-Generation Sequencing (NGS)
Cell-to-Cell Transcription Variability as Measured by Single-Molecule RNA FISH to Detect Epigenetic State Switching
Establishment of Cell Lines Stably Expressing dCas9-Fusions to Address Kinetics of Epigenetic Editing
Editing of DNA Methylation Using dCas9-Peptide Repeat and scFv-TET1 Catalytic Domain Fusions
Chemical Inducible dCas9-Guided Editing of H3K27 Acetylation in Mammalian Cells
Screening Regulatory Element Function with CRISPR/Cas9-based Epigenome Editing