Mengel A., Lindermayr C. (Eds.) Nitric Oxide: Methods and Protocols

Springer Science+Business Media, LLC, 2018. — 297 p. — (Methods in Molecular Biology 1747). — ISBN: 149397694X.This volume explores three main aspects of nitric oxide (NO) research: NO treatment and detection, NO modifications, and NO detoxification. The book also covers methods used to study human/animal and plant nitric oxide. The chapters are divided into three parts: part one looks at NO treatments using gaseous nitric oxide and detection using a NO-sensitive electrode, electron spin resonance, and fluorescence-based NO-sensor proteins.
Part two talks about various techniques used to detect and identify NO-dependent modifications, such as biotin-switch assay and quantification of s-nitrosated proteins. Part three focuses on the study of s-nitrosothiol homeostasis and denitrosation activities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and authoritative, Nitric Oxide: Methods and Protocols is a valuable resource for anyone interested in learning more about this evolving field.NO Treatment and Detection
A Simple and Useful Method to Apply Exogenous NO Gas to Plant Systems: Bell Pepper Fruits as a Model
Measurements of Intra-oocyte Nitric Oxide Concentration Using Nitric Oxide Selective Electrode
Real-Time Imaging of Nitric Oxide Signals in Individual Cells Using geNOps
Detection of Nitric Oxide by Membrane Inlet Mass Spectrometry
Quantum Cascade Lasers-Based Detection of Nitric Oxide
Detection of Nitric Oxide via Electronic Paramagnetic Resonance in Mollusks
NO Modifications
Identification of S-Nitrosylated and Reversibly Oxidized Proteins by Fluorescence Switch and Complementary Techniques
A Proteomics Workflow for Dual Labeling Biotin Switch Assay to Detect and Quantify Protein S-Nitroylation
Surface Plasmon Resonance Spectroscopy for Detection of S-Nitrosylated Proteins
Measurement of S -Nitrosoglutathione in Plasma by Liquid Chromatography–Tandem Mass Spectrometry
Analysis of Recombinant Protein S-Nitrosylation Using the Biotin-Switch Technique
Direct Measurement of S-Nitrosothiols with an Orbitrap Fusion Mass Spectrometer: S-Nitrosoglutathione Reductase as a Model Protein
Identification of Tyrosine and Nitrotyrosine with a Mixed-Mode Solid-Phase Extraction Cleanup Followed by Liquid Chromatography–Electrospray Time-of-Flight Mass Spectrometry in Plants
Electrophoretic Detection and Confocal Microscopic Imaging of Tyrosine Nitrated Proteins in Plant Tissue
Identification of NO-Sensitive Cysteine Residues Using Cysteine Mutants of Recombinant Proteins
Detection of S-Nitrosated Nuclear Proteins in Pathogen-Treated Arabidopsis Cell Cultures Using Biotin Switch Technique
Nitric Oxide Analyzer Quantification of Plant S-Nitrosothiols
Nitro-Fatty Acid Detection in Plants by High-Pressure Liquid Chromatography Coupled to Triple Quadrupole Mass Spectrometry
Bioinformatic Prediction of S-Nitrosylation Sites in Large Protein Datasets
NO Detoxification
Biotin Switch Processing and Mass Spectrometry Analysis of S-Nitrosated Thioredoxin and Its Transnitrosation Targets
Immunodetection of S-Nitrosoglutathione Reductase Protein in Plant Samples
Thioredoxin-Dependent Decomposition of Protein S-Nitrosothiols