Ishii Masaru (ed.) Intravital Imaging of Dynamic Bone and Immune Systems: Methods and Protocols

Humana Press, 2018. — 175 p.
Series: Methods in Molecular Biology, № 1763This detailed volume presents a number of chapters that will aid researchers in jumping the hurdle preventing the popularization of advanced intravital imaging technology usage, namely the complicated experimental protocols. Leading researchers undertaking imaging studies in the field of bone and immune systems have contributed the chapters that aim to guide readers through these complex methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Intravital Imaging of Dynamic Bone and Immune Systems: Methods and Protocols will serve as an idea guide for general readers in order to establish and perform these experiments by their own.Bone Imaging: Osteoclast and Osteoblast Dynamics
Intravital Imaging of Mouse Bone Marrow: Hemodynamics and Vascular Permeability
Bone Imaging: Platelet Formation Dynamics
Live Imaging of Interstitial T Cell Migration Using Lymph Node Slices
Two-Photon Imaging of T-Cell Motility in Lymph Nodes: In Vivo and Ex Vivo Approaches
Imaging the Lymph Node Stroma
Intravital Imaging of B Cell Responses in Lymph Nodes
Live Imaging of the Skin Immune Responses: Visualization of the Contact Hypersensitivity Response
Imaging of Inflammatory Responses in the Mouse Ear Skin
In Vivo Imaging of Immune Cells in Peyer’s Patches
Intravital Imaging of T Cells Within the Spinal Cord
Kidney Imaging: Intravital Microscopy
Intravital Imaging of Liver Cell Dynamics
Intravital Imaging of the Heart at the Cellular Level Using Two-Photon Microscopy
Imaging Window Device for Subcutaneous Implantation Tumor
New Tools for Imaging of Immune Systems: Visualization of Cell Cycle, Cell Death, and Cell Movement by Using the Mice Lines Expressing Fucci, SCAT3.1, and Kaede and KikGR