Xiao W. Yeast Protocols

2nd ed. — Humana Press, 2006. — 406 p. — E-ISBN: 1-59259-958-3
Series: Methods in Molecular Biology, № 313Basic Investigations in Saccharomyces cerevisiae
Isolation of Nucleic Acids
Purification of Yeast Peroxisomes
Isolation of Yeast Plasma Membranes
Isolation of Yeast Mitochondria
Extraction of Yeast Lipids
Two-Dimensional Gel Electrophoresis of Total Yeast Proteins
Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes
Analysis of Yeast Lipids
Yeast Fluorescence Microscopy
Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe
Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
Mutagenesis
Gene Disruption in the Budding Yeast Saccharomyces cerevisiae
Inducible Degron and Its Application to Creating Conditional Mutants
Synthetic Lethal Screen
Synthetic Genetic Array Analysis in Saccharomyces cerevisiae
Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates
Chromatin Assembly in a Crude Fraction From Yeast Cells
Chromatin Immunoprecipitation to Study Protein–DNA Interactions in Budding Yeast
Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning
Study of Transcriptional Regulation Using a Reporter Gene Assay
Assessing Telomeric Phenotypes
Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast
Production of Heterologous Proteins in Yeast With the Aid of the Hsp150∆ Carrier
Yeast Two-Hybrid System Screening
Import of Precursor Proteins Into Isolated Yeast Mitochondria