Carmichael G.G. RNA Silencing: Methods and Protocols

Humana Press, 2005. — 352 p. — (Methods in Molecular Biology 309). — ISBN: 1-59259-935-4.Identification of Components of RNAi Pathways Using the Tandem Affinity Purification Method
Separation of Drosophila RNA Silencing Complexes by Native Gel Electrophoresis
Preparation and Analysis of Drosha
RNA Interference in Caenorhabditis elegans
Down-Regulating Gene Expression by RNA Interference in Trypanosoma brucei
Analysis of Double-Stranded RNA and Small RNAs Involved in RNA-Mediated Transcriptional Gene Silencing
Strategies for the Design of Random siRNA Libraries and the Selection of Anti-GFP siRNAs
Silencing Gene Expression with Dicer-Generated siRNA Pools
Inhibition of Gene Expression In Vivo Using Multiplex siRNA
Gene Silencing by a DNA Vector-Based RNAi Technology
Plasmid-Based RNA Interference: Construction of Small-Hairpin RNA Expression Vectors
siRNA Delivery In Vivo
Peptide-Based Strategy for siRNA Delivery into Mammalian Cells
Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells
Exon-Specific RNA Interference: A Tool to Determine the Functional Relevance of Proteins Encoded by Alternatively Spliced mRNAs
Immunoprecipitation of MicroRNPs and Directional Cloning of MicroRNAs
Detection of MicroRNAs and Assays to Monitor MicroRNA Activities In Vivo and In Vitro
High-Throughput Analysis of MicroRNA Gene Expression Using Sensitive Probes
Modification of Human U1 snRNA for Inhibition of Gene Expression at the Level of Pre-mRNA