Lugnier C. (ed.) Phosphodiesterase Methods and Protocols

Humana Press, 2005 — 337 p. — (Methods in Molecular Biology 307). — ISBN: 1-59259-901-X.Study of Cyclic Adenosine Monophosphate Microdomains in Cells
High-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D Microdomains
Cygnets: In Vivo Characterization of Novel cGMP Indicators and In Vivo Imaging of Intracellular cGMP
High-Throughput Screening of Phosphodiesterase Activity in Living Cells
Assessment of Phosphodiesterase Isozyme Contribution in Cell and Tissue Extracts
Localization of the Cyclic Guanosine 3',5'-MonophosphateHydrolyzing Phosphodiesterase Type 9 in Rat Brain by Nonradioactive In Situ Hybridization
Determination of Ca2+/Calmodulin-Stimulated Phosphodiesterase Activity in Intact Cells
Adenovirus-Mediated Overexpression of Murine Cyclic Nucleotide Phosphodiesterase 3B
Identification of Promoter Elements in the 5'-Flanking Region of Murine Cyclic Nucleotide Phosphodiesterase 3B Gene
Purification of PDE6 Isozymes From Mammalian Retina
Cyclic Guanosine 5'-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor Phosphodiesterase
Renaturation of the Catalytic Domain of PDE4A Expressed in Escherichia coli as Inclusion Bodies
Determining the Subunit Structure of Phosphodiesterases Using Gel Filtration and Sucrose Density Gradient Centrifugation
Crystallization of Cyclic Nucleotide Phosphodiesterases
Generation of PDE4 Knockout Mice by Gene Targeting
Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase
Investigation of Extracellular Signal-Regulated Kinase 2 Mitogen-Activated Protein Kinase Phosphorylation and Regulation of Activity of PDE4 Cyclic Adenosine Monophosphate-Specific Phosphodiesterases
Radiolabeled Ligand Binding to the Catalytic or Allosteric Sites of PDE5 and PDE11
Analysis of Dimerization Determinants of the PDE6 Catalytic Subunits
Interaction Between Catalytic and Inhibitory Subunits of PDE6
Purification, Reconstitution on Lipid Vesicles, and Assays of PDE6 and Its Activator G Protein, Transducin