Patterson C., Cyr D.M. Ubiquitin-Proteasome Protocols

Humana Press, 2005. — 392 p. — (Methods in Molecular Biology 301). — eISBN 1-59259-895-1.Biochemical Methods to Study the Ubiquitin–Proteasome System
Small-Molecule Inhibitors of Proteasome Activity
Purification of E1 and E1-Like Enzymes
Assays for RING Family Ubiquitin Ligases
Ubiquitin Chain Synthesis
Purification of Proteasomes, Proteasome Subcomplexes, and Proteasome-Associated Proteins From Budding Yeast
Recognition and Processing of Misfolded Proteins by PA700, the 19S Regulatory Complex of the 26S Proteasome
Cell-Free Assay for Ubiquitin-Independent Proteasomal Protein Degradation
Assays of Proteasome-Dependent Cleavage Products
Identification of Components of Protein Complexes
Mass Spectrometric Determination of Protein Ubiquitination
Reconstitution of Endoplasmic Reticulum-Associated Degradation Using Yeast Membranes and Cytosol
Reticulocyte Lysate as a Model System to Study Endoplasmic Reticulum Membrane Protein Degradation
Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterization
Cellular Methods to Study Ubiquitin–Proteasome-Dependent Functions
Measuring Ubiquitin Conjugation in Cells
Assays for Proteasome Assembly and Maturation
N-Terminal Ubiquitination
Quantitating Defective Ribosome Products
Endoplasmic Reticulum-Associated Protein Quality Control and Degradation: Screen for ERAD Mutants After Ethylmethane Sulfonate Mutagenesis
Endoplasmic Reticulum-Associated Protein Quality Control and Degradation: Genome Wide Screen for ERAD Components
Cystic Fibrosis Transmembrane Conductance Regulator as a Model Substrate to Study Endoplasmic Reticulum Protein Quality Control in Mammalian Cells
Aggresome Formation
Detection of Sumoylated Proteins
Proteasome Inhibitors in Cancer Therapy
Parkinson’s Disease: Assays for the Ubiquitin Ligase Activity of Neural Parkin