Peña L. Transgenic Plants: Methods and Protocols

Humana Press, 2005 — 452 p. — eISBN 1-59259-827-7
Series: Methods in Molecular Biology, № 286Transgenic Plants: An Historical Perspective
Transformation
Plant Transformation: Agrobacterium-Mediated Gene Transfer
Production Of Hairy Root Cultures And Transgenic Plants By Agrobacterium Rhizogenes-Mediated Transformation
Stable Transformation Of Plant Cells By Particle Bombardment/Biolistics
Electroporation: Introduction And Expression Of Transgenes In Plant Protoplasts
Floral Dip: Agrobacterium-Mediated Germ Line Transformation
Production Of Transgenic Crops By The Floral-Dip Method
Chloroplast Genetic Engineering To Improve Agronomic Traits
Regeneration
Organogenesis From Transformed Tomato Explants
Genetic Transformation Of Conifers Utilizing Somatic Embryogenesis
Regeneration Of Transgenic Cassava From Transformed Embryogenic Tissues
Genetic Transformation Of Mature Citrus Plants
Selection
Selectable Markers: Antibiotic And Herbicide Resistance
Histochemical And Fluorometric Assays For Uida (Gus) Gene Detection
Green Fluorescent Protein Quantification In Whole Plants
Positive Selection
Elimination Of Marker Genes From Transgenic Plants Using Mat Vector Systems
Simple And Efficient Removal Of Marker Genes From Plastids By Homologous Recombination
Transgene Integration, Expression, And Localization
The Study Of Transgene Copy Number And Organization
Analysis Of Gene Expression In Transgenic Plants
Transgene Integration: Use Of Matrix Attachment Regions
Fluorescence In Situ Hybridization To Localize Transgenes In Plant Chromosomes
Amplification Of Genomic Sequences Flanking T-DNA Insertions By Thermal Asymmetric Interlaced Polymerase Chain Reaction
Risk Assessment
Agrobacterium Persistence In Plant Tissues After Transformation
Transgene Dispersal Through Pollen
Transgenic Crops
Transgenic Crops: The Current And Next Generations
Comparative Development And Impact Of Transgenic Papayas In Hawaii, Jamaica, And Venezuela