Kohwi Y. Trinucleotide Repeat Protocols

Humana Press, 2004 — 355 p. — e-ISBN: 1-59259-804-8
Series: Methods in Molecular Biology, № 277Mouse Models of Triplet Repeat Diseases
Analysis of triplet repeat DNAs and RNAs
Analysis of Triplet Repeat Replication by Two-Dimensional Gel Electrophoresis
Genetic Analysis for Triplet Repeat Instability in Yeast
Detection and Isolation of Trinucleotide Repeat Expansions Using the RED Method
Analysis of Unstable Triplet Repeats Using Small-Pool Polymerase Chain Reaction
Real-Time RT-PCR for CTG Repeat-Containing Genes
Detection and analysis of polyglutamine-containing proteins and their aggregates
Antibodies Against Huntingtin: Production and Screening of Monoclonals and Single-Chain Recombinant Forms
Using Antibodies to Analyze Polyglutamine Stretches
Solubilization of Aggregates Formed by Expanded Polyglutamine Tract Expression in Cultured Cells
Establishment of animal and cultured cell models for trinucleotide repeat diseases
Caenorhabditis elegans as a Model System for Triplet Repeat Diseases
Monitoring Aggregate Formation in Organotypic Slice Cultures
The CGG Repeat and the FMR1 Gene
Analysis of CTG Repeats Using DM1 Model Mice
Lentiviral-Mediated Gene Transfer to Model Triplet Repeat Disorders
Mouse Tissue Culture Models of Unstable Triplet Repeats
In vivo analysis of trinucleotide repeat diseases
Neurotransmitter Receptor Analysis in Transgenic Mouse Models
Chromatin Immunoprecipitation Technique for Study of Transcriptional Dysregulation in Intact Mouse Brain
Techniques for Thick-Section Golgi Impregnation of Formalin-Fixed Brain Tissue
Assessment of Impaired Proteasomal Function in a Cellular Model of Polyglutamine Diseases
Assessment of In Vitro and In Vivo Mitochondrial Function in Friedreich’s Ataxia and Huntington’s Disease
Triplet Repeats and DNA Repair: Germ Cell and Somatic Cell Instability in Transgenic Mice
Oxidative Damage in Huntington’s Disease