Melville S.E. Parasite Genomics Protocols

Humana Press, 2004 — 467 p. — e-ISBN: 1-59259-793-9
Series: Methods in Molecular Biology, № 270Sequencing Strategies for Parasite Genomes
Annotation of Parasite Genomes
Parasite Genome Databases and Web-Based Resources
Expressed Sequence Tags: Medium-Throughput Protocols
Expressed Sequence Tags: Analysis and Annotation
Positive Selection Scanning of Parasite DNA Sequences
Amplified (Restriction) Fragment Length Polymorphism (AFLP) Analysis
Minisatellites and MVR-PCR for the Individual Identification of Parasite Isolates
Analysis of Differentially Expressed Parasite Genes and Proteins Using Transcriptomics and Proteomics
Gene Expression Studies Using Self-Fabricated Parasite cDNA Microarrays
DNA Content Analysis on Microarrays
Typing Single-Nucleotide Polymorphisms in Toxoplasma gondii by Allele-Specific Primer Extension and Microarray Detection
Transfection of the Human Malaria Parasite Plasmodium falciparum
A PCR-Based Method for Gene Deletion and Protein Tagging in Trypanosoma brucei
Analysis of Gene Function in Trypanosoma brucei Using RNA Interference
In Vitro Shuttle Mutagenesis Using Engineered Mariner Transposons
Random Mutagenesis Strategies for Construction of Large and Diverse Clone Libraries of Mutated DNA Fragments
Separation, Digestion, and Cloning of Intact Parasite Chromosomes Embedded in Agarose
Chromosome Fragmentation in Leishmania
FISH Mapping for Helminth Genomes
Fiber-FISH: Fluorescence In Situ Hybridization on Stretched DNA
Yeast Two-Hybrid Assay for Studying Protein–Protein Interactions
From Genomes to Vaccines for Leishmaniasis