2 nd ed. — Humana Press, 2004. — 523 P. — E-ISBN: 1-59259-774-2.
General issues about recombinant gene expression.
Host Cell Compatibility in Protein Expression.
Production of Recombinant Proteins: Challenges and Solutions.
Folding-Promoting Agents in Recombinant Protein Production.
Prokaryotes.
Back to Basics: pBR322 and Protein Expression Systems in E.сoli.
α-Complementation Enabled T7 expression Vectors and Their Use for the Expression of Recombinant Polypeptides for Protein Transduction Experiments.
Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli.
Overexpression of Chromosomal Genes in Escherichia coli.
Chromosomal Expression of Foreign and Native Genes From Regulatable Promoters in Escherichia coli.
Plasmid Vectors for Marker-Free Chromosomal Insertion of Genetic Material in Escherichia coli.
Copy-Control pBAC/oriV Vectors for Genomic Cloning.
Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriV.
Cell-Free Protein Synthesis With Prokaryotic Combined Transcription-Translation.
Genetic Tools for the Manipulation of Moderately Halophilic Bacteria of the Family Halomonadaceae.
Gene Transfer and Expression of Recombinant Proteins in Moderately Halophilic Bacteria.
Recombinant Protein Production in Antarctic Gram-Negative Bacteria.
Fungi.
Recombinant Protein Production in Yeasts.
Controlled Expression of Homologous Genes by Genomic Promoter Replacement in the Yeast Saccharomyces cerevisiae.
High-Throughput Expression in Microplate Format in Saccharomyces cerevisiae.
High-Throughput Expression in Microplate Format in Pichia pastoris.
Multiple Gene Expression by Chromosomal Integration and CRE-loxP-Mediated Marker Recycling in Saccharomyces cerevisiae.
Three Decades of Fungal Transformation: Key Concepts and Applications.
Three Decades of Fungal Transformation: Novel Technologies.
Plants and plant cells.
Gene Transfer and Expression in Plants.
Production of Recombinant Proteins by Hairy Roots Cultured in Plastic Sleeve Bioreactors.
Engineering the Chloroplast Genome for Hyperexpression of Human Therapeutic Proteins and Vaccine Antigens.
New Selection Marker for Plant Transformation.
Enhancer Detection and Gene Trapping as Tools for Functional Genomics in Plants.
Animals and animal cells.
Gene Transfer and Expression in Mammalian Cell Lines and Transgenic Animals.
Sustained Heterologous Transgene Expression in Mammalian and Avian Cell Lines.
Inducible Gene Expression in Mammalian Cells and Mice.
Flp-Mediated Integration of Expression Cassettes into Frt-tagged Chromosomal Loci in Mammalian Cells.
Generation of High-Recombinant Protein-Producing Chinese Hamster Ovary (CНО) cells.
Preparation of Recombinant Proteins in Milk.