Domingues L. (Ed.) PCR: Methods and Protocols

Springer Science+Business Media LLC, 2017. — 283 p. — (Methods in Molecular Biology 1620) — ISBN10: 1493970593.This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field. Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology, and healthcare. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.In Silico PCR Tools for a Fast Primer, Probe, and Advanced Searching
Introduction on Using the FastPCR Software and the Related Java Web Tools for PCR and Oligonucleotide Assembly and Analysis
Long Fragment Polymerase Chain Reaction
Strategies to Improve Efficiency and Specificity of Degenerate Primers in PCR
Inverse PCR for Point Mutation Introduction
Synthesis of Fusion Genes for Cloning by Megaprimer-Based PCR
A Novel Platform for High-Throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli
Colony PCR
CRENAME, A Molecular Microbiology Method Enabling Multiparametric Assessment of Potable/Drinking Water
Multiplex Detection of Food-Borne Pathogens
Fast Real-Time PCR for the Detection of Crustacean Allergens in Foods
Fast Real-Time PCR Method for Detection of Soy in Foods
RAPD/SCAR Approaches for Identification of Adulterant Breeds Milk in Dairy Products
Genetic Diversity Analysis of Medicinally Important Horticultural Crop Aegle marmelos by ISSR Markers
PCR in the Analysis of Clinical Samples: Prenatal and Postnatal Diagnosis of Inborn Errors of Metabolism
Harnessing the Power of PCR Molecular Fingerprinting Methods and Next Generation Sequencing for Understanding Structure and Function in Microbial Communities
PCR in Metagenomics
Arbitrarily Primed PCR for Comparison of Meta Genomes and Extracting Useful Loci from Them