Espina V. (Ed.) Molecular Profiling: Methods and Protocols

2nd Edition. — Springer Science+Business Media LLC, 2017. — 412 p. — (Methods in Molecular Biology 1606) — ISBN: 9781493969890.This second edition provides an update on technology and an accelerated tutorial to assist, students, entrepreneurs, new investigators, and established investigators who want to quickly become versed in, and immersed in, the entire process from discovery to clinical trial validation and commercial public benefit. Chapters aim to cover the full spectrum of molecular profiling from tumor staging and grading through biomarker discovery, to commercialization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Molecular Profiling: Methods and Protocols, Second Edition aims to be a useful guide on molecular profiling.Tumor Staging and Grading: A Primer
Innovations in Clinical Trial Design in the Era of Molecular Profiling
Personalized Medicine: Ethical Aspects
Antibody Validation by Western Blotting
Scanning Electron Microscopy Sample Preparation and Imaging
One-Step Preservation and Decalcification of Bony Tissue for Molecular Profiling
Application of Hydrogel Nanoparticles for the Capture, Concentration, and Preservation of Low-Abundance Biomarkers
Using Laser Capture Microdissection to Isolate Cortical Laminae in Nonhuman Primate Brain
Western Blot Techniques
ELISA for Monitoring Nerve Growth Factor
Reverse Phase Protein Microarrays
Clustering and Network Analysis of Reverse Phase Protein Array Data
PCR: Identification of Genetic Polymorphisms
Microsatellite Analysis for Identification of Individuals Using Bone from the Extinct Steller’s Sea Cow (Hydrodamalis gigas)
Somatic DNA Mutation Analysis
Optimization of Immunostaining for Prospective Image Analysis
Fluorescence In Situ Hybridization of Cells, Chromosomes, and Formalin-Fixed Paraffin-Embedded Tissues
High-Resolution Image Stitching as a Tool to Assess Tissue-Level Protein Distribution and Localization
Mass Spectrometry-Based Biomarker Discovery
Quantitative Mass Spectrometry by Isotope Dilution and Multiple Reaction Monitoring (MRM)
LC-Mass Spectrometry for Metabolomics
Metabolomic Bioinformatic Analysis
Stable Isotope Quantitative N-Glycan Analysis by Liquid Separation Techniques and Mass Spectrometry
Grant Writing Tips for Translational Research
Inventions and Patents: A Practical Tutorial
Product Development and Commercialization of Diagnostic or Life Science Products for Scientists and Researchers