D’Ambrosio D., Sinigaglia F. (Eds.) Cell Migration in Inflammation and Immunity: Methods and Protocols

Humana Press, 2003. — 269 p.
Series: Methods in Molecular Biology, № 239Cell migration is now well recognized as a critical component of the inflammatory disease process, so that its proper understanding promises to generate both ground-breaking basic discoveries and the development of novel therapeutics. In this volume leading cell biologists and immunologists present their most widely useful and innovative techniques for studying the molecular and cellular basis of this phenomenon. Describing each method in step-by-step detail, the authors provide a series of focused, cutting-edge techniques proceeding from the in vitro analysis of cell migration and the molecular mechanisms underlying this process, to methodologies for the analysis of cell migration in vivo. Methods for the analysis of rapid leukocyte adhesion under flow conditions in vitro are described, which may prove especially fruitful for scientists exploring the molecular mechanisms underlying both vascular recognition and leukocyte-endothelium interaction. Experimental approaches useful in establishing the role of cell migration in the pathogenesis of both acute and chronic inflammatory diseases are emphasized. Each fully tested protocol includes an introduction explaining the principle behind the technique, equipment and reagent lists, and tips on troubleshooting and how to avoid known pitfalls.