Engelborghs Y., Visser A.J.W.G. (Eds.) Fluorescence Spectroscopy and Microscopy: Methods and Protocols

New York: Humana Press, 2014. — 812 p. — (Methods in Molecular Biology 1076).Reflecting the expanding field’s need for reliable protocols, this volume offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.