Dumitrescu R.G., Verma M. (Eds.) Cancer Epigenetics: Methods and Protocols

New York: Humana Press, 2012. — 492 p. — (Methods in Molecular Biology 863). — ISBN: 978-1-61779-611-1, 978-1-61779-612-8.Includes epigenetic alterations that can be use in clinical practice
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the expertsThe epigenetic regulation plays an important role in normal development and maintenance of tissue specific genes expression in humans and the disturbance of these patterns lead to changes involved in tumor formation. More recently, epigenetic changes have been observed in early stages of tumor development and together with the genetic alterations have been defined as abnormalities, necessary for cancer initiation and progression. In, Cancer Epigenetics: Methods and Protocols, expert researchers reviewed these epigenetics changes in different tumor types and described several technologies that are currently available to detect epigenetic changes. These technologies have lead to a better understanding of the processes in normal and cancerous cells. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Thorough and intuitive, Cancer Epigenetics: Methods and Protocols aids scientists in continuing to study epigenetic alterations used in clinical practice as biomarkers of early cancerous lesions or markers of progression and prognosis.Epigenetic Markers of Early Tumor Development
Epigenetics of Solid Cancer Stem Cells
DNA Methylation and Histone Modifications in Breast Cancer
DNA Methylation Changes in Prostate Cancer
DNA Methylation in Promoter Region as Biomarkers in Prostate Cancer
Epigenetics of Bladder Cancer
Epigenetics in Myeloid Malignancies
The Epigenetics of Brain Tumors
DNA Methylation Changes in Cervical Cancers
Epigenetics of Colon Cancer
Promoter Methylation in Head and Neck Tumorigenesis
Epigenome and DNA Methylation in Oral Squamous Cell Carcinoma
Clinical Implications of Epigenetic Alterations in Human Thoracic Malignancies: Epigenetic Alterations in Lung Cancer
The Role of MicroRNAs in the Management of Liver Cancer
Epigenetics in Ovarian Cancer
DNA Methylation in Pancreatic Cancer: Protocols for the Isolation of DNA and Bisulfite Modification
Detection and Analysis of DNA Methylation by Pyrosequencing
Screening for miRNA Expression Changes Using Quantitative PCR (Q-PCR)
Genome-Wide Methylation Analysis
Microarray for Epigenetic Changes: Gene Expression Arrays
Detecting DNA Methylation Using the Methylated CpG Island Amplification and Microarray Technique
Nutrigenomics: Implications for Breast and Colon Cancer Prevention
Dietary and Lifestyle Factors of DNA Methylation
Diet, Epigenetics, and Cancer
Role of Epigenetics in Cancer Health Disparities
Multifactorial Etiology of Gastric Cancer
Epigenetic Epidemiology for Cancer Risk: Harnessing Germline Epigenetic Variation
Epigenetic Biomarkers in Cancer Epidemiology
Screening for miRNA Expression Changes Using Quantitative PCR (Q-PCR)