Bosselut R., Vacchio M.S. (Eds.) T-Cell Development: Methods and Protocols

Humana Press, 2016. — 311 p.
This volume provides simple and accessible experiment protocols to explore thymus biology. The book is divided into three parts presenting short reviews on T cell development, analysis strategies, protocols for cell preparation, flow cytometry analyses, and multiple aspects of thymocyte biology. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.