Pellicciari C., Biggiogera M. (Eds.) Histochemistry of Single Molecules: Methods and Protocols

Springer Science+Business Media LLC, 2017. — 335 p. — (Methods in Molecular Biology 1560) — ISBN: 1493967878The story of histochemistry goes a long way back and, in the last 150 years, has evolved in parallel with the operating instruments, from bright field microscopy to super-resolution microscopy. In this evolution resides the uniqueness of this science. Compared to the biochemical and molecular methods, the distinctive feature of histochemical techniques is the ability to localize chemical species in the very place (in a tissue, or a cell or an organelle) where they exist, are produced, or operate in vivo; this makes histochemistry an irreplaceable tool in basic and applied biomedical research.Vital Histochemistry
Single Cell Cytochemistry Illustrated by the Demonstration of Glucose-6-Phosphate Dehydrogenase Deficiency in Erythrocytes
Autofluorescence Spectroscopy for Monitoring Metabolism in Animal Cells and Tissues
Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree
Histochemical Demonstration of Tripeptidyl Aminopeptidase I
Enzyme Histochemistry for Functional Histology in Invertebrates
Lectin Histochemistry
Lectin Histochemistry: Historical Perspectives, State of the Art, and the Future
Isolation of Viable Glycosylation-Specific Cell Populations for Further In Vitro or In Vivo Analysis Using Lectin-Coated Magnetic Beads
Lectin Histochemistry for Metastasizing and Non-metastasizing Cancer Cells
The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium
Protein Histochemistry
Heat-Induced Antigen Retrieval in Immunohistochemistry: Mechanisms and Applications
Detecting Neuronal Differentiation Markers in Newborn Cells of the Adult Brain
Characterizing Satellite Cells and Myogenic Progenitors During Skeletal Muscle Regeneration
Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin- Embedded Tissue
Lipid Histochemistry
Tissue Fixation and Processing for the Histological Identification of Lipids
Staining Methods for Normal and Regenerative Myelin in the Nervous System
Nile Red Staining of Neutral Lipids in Yeast
Staining of Lipid Droplets with Monodansylpentane
Nuclear HistochemIstry
Fluorochromes for DNA Staining and Quantitation
Osmium Ammine for Staining DNA in Electron Microscopy
DNA Labeling at Electron Microscopy
Visualizing RNA at Electron Microscopy by Terbium Citrate
Two-Tailed Comet Assay (2T-Comet): Simultaneous Detection of DNA Single and Double Strand Breaks
Plant Histochemistry
Detection of Endogenous Nuclear Proteins in Plant Cells: Localizing Nuclear Matrix Constituent Proteins (NMCPs), the Plant Analogs of Lamins
Histochemical Analysis of Plant Secretory Structures
A Histochemical Technique for the Detection of Annonaceous Acetogenins