Keppler D., Lin A.W. (Eds.) Cervical Cancer: Methods and Protocols

Humana Press, 2015. — 413 p. — (Methods in Molecular Biology 1249). — ISBN: 149392012X, 9781493920129.Includes cutting-edge methods and protocols for researching cervical cancer and high-risk HPV types
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the expertsRepresenting the most relevant procedures and technologies aiding the advance of the field of HPV-mediated carcinogenesis of the cervix and other anatomical regions of squamocolumnar transition, such as the anorectum, penis, and oropharynx, Cervical Cancer: Methods and Protocols compiles a detailed collection of practical chapters. The first half of the book covers HPV types, pathogenesis of cervical cancer (CxCA), prevention, and novel potential drug targets, while the second half explores pathology, genomics, modeling of CxCA, and experimental therapeutic strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and vital, Cervical Cancer: Methods and Protocols serves as a valuable resource to both bench scientists and clinicians who step into the realm of high-risk HPVs and CxCA for the first time or those who wish to learn novel approaches or expand their toolbox for the study of CxCA.Evolution and Classification of Oncogenic Human Papillomavirus Types and Variants Associated with Cervical Cancer
A Real-Time PCR Approach Based on SPF10 Primers and the INNO-LiPA HPV Genotyping Extra Assay for the Detection and Typing of Human Papillomavirus
Replication of Human Papillomavirus in Culture
HPV Binding Assay to Laminin-332/Integrin α6β4 on Human Keratinocytes
Methods to Assess the Nucleocytoplasmic Shuttling of the HPV E1 Helicase and Its Effects on Cellular Proliferation and Induction of a DNA Damage Response
Genetic Methods for Studying the Role of Viral Oncogenes in the HPV Life Cycle
Robust HPV-18 Production in Organotypic Cultures of Primary Human Keratinocytes
A High-Throughput Cellular Assay to Quantify the p53-Degradation Activity of E6 from Different Human Papillomavirus Types
Retroviral Expression of Human Cystatin Genes in HeLa Cells
Molecular Analysis of Human Papillomavirus Virus-Like Particle Activated Langerhans Cells In Vitro
Selective Silencing of Gene Target Expression By siRNA Expression Plasmids in Human Cervical Cancer Cells
Silencing of E6/E7 Expression in Cervical Cancer Stem-Like Cells
Two-Step Procedure for Evaluating Experimentally Induced DNA Damage: Texas Red and Comet Assays
Measurement of Deubiquitinating Enzyme Activity Via a Suicidal HA-Ub-VS Probe
Immunocytochemical Analysis of the Cervical Pap Smear
Diagnosis of HPV-Negative, Gastric-Type Adenocarcinoma of the Endocervix
Targeting of the HPV-16 E7 Protein by RNA Aptamers
The Use of MYBL2 as a Novel Candidate Biomarker of Cervical Cancer
Fixation Methods for the Preservation of Morphology, RNAs, and Proteins in Paraffin-Embedded Human Cervical Cancer Cell Xenografts in Mice
Assessment of the HPV DNA Methylation Status in Cervical Lesions
MeDIP-on-Chip for Methylation Profiling
Use of DBD-FISH for the Study of Cervical Cancer Progression
A Quantitative and High-Throughput Assay of Human Papillomavirus DNA Replication
Native Human Papillomavirus Production, Quantification, and Infectivity Analysis
Functional Analysis of HPV-Like Particle-Activated Langerhans Cells In Vitro
Assessment of the Radiation Sensitivity of Cervical Cancer Cell Lines
Mouse Model of Cervicovaginal Papillomavirus Infection
Establishment of Orthotopic Primary Cervix Cancer Xenografts
Generation of K14-E7/∆N87βcat Double Transgenic Mice as a Model of Cervical Cancer