Strano S. (Ed.) Cancer Chemoprevention: Methods and Protocols

New York: Humana Press, 2016. — 214 p. — (Methods in Molecular Biology 1379). — ISBN: 978-1-4939-3190-3, 978-1-4939-3191-0.Despite the advent of personalized therapies, cancer still remains a leading cause of death worldwide. The number of cancer cases is rising yearly and is expected to double in the next 20 years. The largest increase of new cases will occur mainly in lower income countries, where the access to cancer care is still inadequate. Thus, there is a strong and urgent need of additional and complementary therapies. The increasing healthcare costs of novel anticancer therapeutic agents and their toxic side effects in high-income countries pave the way for cancer chemoprevention strategies. Natural compounds and dietary supplement phytochemicals appear to be effi cacious arms of intervention in cancer chemoprevention.The potential value of these agents has been demonstrated in preclinical and observational studies. Large-scale clinical trials of primary and secondary cancer chemoprevention are still in progress. As for any type of clinical trials, the monitoring of the endpoints through the evaluation of proper biomarkers is still the challenge to pursue. With the present book for Cancer Chemoprevention Protocols, we depict along the 18 chapters the state of the art of methods that can be useful for both basic and translational researchers to conduct chemoprevention preclinical studies. Each chapter includes an introduction to the specifi c technology and a detailed method section. Published and unpublished observations of the contributing authors are also included.Controlled Delivery of Chemopreventive Agents by Polymeric Implants
Use of Buffy Coat miRNA Profiling for Breast Cancer Prediction in Healthy Women
microRNAs in Cancer Chemoprevention: Method to Isolate Them from Fresh Tissues
Application of RNA-Seq Technology in Cancer Chemoprevention
Detection of Circulating Tumor DNA in the Blood of Cancer Patients: An Important Tool in Cancer Chemoprevention
The Methylated DNA Immunoprecipitation [MeDIP] to Investigate the Epigenetic Remodeling in Cell Fate Determination and Cancer Development
LC-MS-Based Metabolomic Investigation of Chemopreventive Phytochemical-Elicited Metabolic Events
1H NMR Metabolomic Footprinting Analysis for the In Vitro Screening of Potential Chemopreventive Agents
Comet Assay in Cancer Chemoprevention
Angiogenesis Assays
AlgiMatrix-Based 3D Cell Culture System as an In Vitro Tumor Model: An Important Tool in Cancer Research
Cancer Gastric Chemoprevention: Isolation of Gastric Tumor-Initiating Cells
Isolation of Chemoresistant Cell Subpopulations
Autophagy in Cancer Chemoprevention: Identification of Novel Autophagy Modulators with Anticancer Potential
Protocol for a Steady-State FRET Assay in Cancer Chemoprevention
3D Tumor Models and Time-Lapse Analysis by Multidimensional Microscopy
Antibody Array as a Tool for Screening of Natural Agents in Cancer Chemoprevention
South African Herbal Extracts as Potential Chemopreventive Agents: Screening for Anticancer Splicing Activity