Kovalchuck I. (Ed.) Plant Epigenetics: Methods and Protocols

2nd Edition. — Springer Science+Business Media, New York, 2017. — 256 p. — (Methods in Molecular Biology 1456) — ISBN10: 1489977066This volume provides a variety of protocols to analyze various epigenetic changes, including differential expression of non-coding RNAs, changes in DNA methylation, and histone modifications in plants. Chapters detail protocols with different degrees of complexity, and describe bioinformatics approaches for data processing and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Plant Epigenetics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis
Chromatin Conformation Capture-Based Analysis of Nuclear Architecture
Meta-analysis of Genome-Wide Chromatin Data
Localization of miRNAs by In Situ Hybridization in Plants Using Conventional Oligonucleotide Probes
The Combined Bisulfite Restriction Analysis (COBRA) Assay for the Analysis of Locus-Specific Changes in Methylation Patterns
Analysis of Global Genome Methylation Using the Cytosine- Extension Assay
In Situ Analysis of DNA Methylation in Plants
Analysis of DNA Hydroxymethylation Using Colorimetric Assay
Analysis of DNA Cytosine Methylation Patterns Using Methylation-Sensitive Amplification Polymorphism (MSAP)
Differentially Methylated Region-Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify DMRs in Uncharacterized Genomes
Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays
Northern Blotting Techniques for Small RNAs
Stem-Loop qRT-PCR for the Detection of Plant microRNAs
Profiling New Small RNA Sequences
Small RNA Library Preparation and Illumina Sequencing in Plants
Bioinformatics Analysis of Small RNA Transcriptomes: The Detailed Workflow
Increasing a Stable Transformation Efficiency of Arabidopsis by Manipulating the Endogenous Gene Expression Using Virus-Induced Gene Silencing
The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA Strand Breaks
Profiling Transposable Elements and Their Epigenetic Effects in Non-model Species