Dakubo G.D. Cancer Biomarkers in Body Fluids: Principles

Springer International Publishing, Switzerland, 2016. — 329 p. — ISBN: 3319015796The molecular and genetic signatures of cancer are represented in the peripheral circulation and other body fluids, giving rise to the “liquid biopsy” concept. This new paradigm of molecular profiling of cancer cells offers several advantages over traditional tissue biopsy. It is convenient, noninvasive, conforms to current clinical practice, enables real time disease monitoring and the study of tumor evolution, can easily be sampled multiple times, and this sample is more representative of the heterogeneous cancer cells than biopsy sampling. Indeed, all aspects of cancer molecular genetic information, stemming from DNA (both nuclear and mitochondria), RNA (coding and noncoding), peptides and proteins, metabolites and lipids are present in body fluids as free, cell surface bound or enclosed in membrane vesicles, and are being harnessed for disease management. Additionally, circulating tumor, and tumor stem cells provide prognostic information, and also enable the study of the intricate molecular processes associated with metastasis and drug resistance. This treatise deals with the general principles of the molecular pathology of cancer, and its associated imprints in circulation. The transitional process from discovery, prototype development, translational research, to product development can be complex and costly. The critical path to biomarker development and qualification for successful use in drug development is detailed herein as well.
This book is of interest to Cancer Researchers, Oncologists, Clinicians, Surgeons, Medical Students, Nurses, Diagnostic Laboratories, and Pharmaceutical Industries.Molecular Pathology of Cancer
Advanced Technologies for Body Fluid Biomarker Analyses
Liquid Biopsy and Cancer Biomarkers
Methylated DNA as Cancer Biomarkers in Circulation
Nucleic Acids as Cancer Biomarkers in Circulation
Noncoding Transcripts as Cancer Biomarkers in Circulation
Mitochondrial Genome Changes as Cancer Biomarkers in Circulation
Circulating Tumor Cells and Cancer Stem Cells
Extracellular Vesicles as Cancer Biomarkers in Circulation
The Critical Path to Successful Biomarker Development
Ethical Considerations in Biomarker Development