Matthiesen R. (Ed.) Proteostasis: Methods and Protocols

Humana Press, 2016. — 505 p. — ISBN: 1493937545
Series: Methods in Molecular Biology, №1449This volume highlights the role of proteostasis in human health and associated disease model systems, reflecting its rising importance which has led to the development of new technologies to obtain insight into underling protein mechanistic events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Proteostasis: Methods and Protocols aims to become a reference book on proteostasis in human health.UPS Activation in the Battle Against Aging and Aggregation-Related Diseases: An Extended Review
Review and Literature Mining on Proteostasis Factors and Cancer
Combining Zebrafish and Mouse Models to Test the Function of Deubiquitinating Enzyme (Dubs) Genes in Development: Role of USP45 in the Retina
Immunodepletion and Immunopurification as Approaches for CSN Research
Studying Protein Ubiquitylation in Yeast
Strategies to Detect Endogenous Ubiquitination of a Target Mammalian Protein
In Vitro Ubiquitination: Self-Ubiquitination, Chain Formation, and Substrate Ubiquitination Assays
8 Isolation of the Ubiquitin-Proteome from Tumor Cell Lines and Primary Cells Using TUBEs
TUBEs-Mass Spectrometry for Identification and Analysis of the Ubiquitin-Proteome
Isolation of Ubiquitinated Proteins to High Purity from In Vivo Samples
Method for the Purification of Endogenous Unanchored Polyubiquitin Chains
Fluorescent Tools for In Vivo Studies on the Ubiquitin- Proteasome System
Bimolecular Fluorescence Complementation to Assay the Interactions of Ubiquitylation Enzymes in Living Yeast Cells
Monitoring Ubiquitin-Coated Bacteria via Confocal Microscopy
Detection and Analysis of Cell Cycle-Associated APC/C-Mediated Cellular Ubiquitylation In Vitro and In Vivo
Detection and Analysis of SUMOylation Substrates In Vitro and In Vivo
Detection of Protein–Protein Interactions and Posttranslational Modifications Using the Proximity Ligation Assay: Application to the Study of the SUMO Pathway
Dissecting SUMO Dynamics by Mass Spectrometry
Isolation of Lysosomes from Mammalian Tissues and Cultured Cells
Analysis of Relevant Parameters for Autophagic Flux Using HeLa Cells Expressing EGFP-LC3
Analysis of Protein Oligomeric Species by Sucrose Gradients
Analysis of Protein Oligomerization by Electrophoresis
Blot-MS of Carbonylated Proteins: A Tool to Identify Oxidized Proteins
Quantitation of Protein Translation Rate In Vivo with Bioorthogonal Click-Chemistry
A Simple Protocol for High Efficiency Protein Isolation After RNA Isolation from Mouse Thyroid and Other Very Small Tissue Samples
Monitoring Target Engagement of Deubiquitylating Enzymes Using Activity Probes: Past, Present, and Future
Activity Based Profiling of Deubiquitylating Enzymes and Inhibitors in Animal Tissues
High-Throughput siRNA Screening Applied to the Ubiquitin–Proteasome System
High-Throughput Yeast-Based Reporter Assay to Identify Compounds with Anti-inflammatory Potential
Using AlphaScreen to Identify Small-Molecule Inhibitors Targeting a Conserved Host–Pathogen Interaction
Global MS-Based Proteomics Drug Profiling