Németh A.(Ed.) The Nucleolus: Methods and Protocols

Humana Press, 2016. — 293 p. — ISBN: 1493937901
Series: Methods in Molecular Biology, № 1455This volume provides an up-to-date compilation of current methodological approaches utilized for the exploration of nucleolar structure and function. Chapters cover a diversity of protocols that include imaging of the nucleolus, analysis of ribosomal RNA transcription and processing, and genomics and proteomics of the nucleolus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Nucleolus: Methods and Protocols provides scientists with a reliable practical handbook to facilitate the investigation of this nuclear compartment at the advanced level.Imaging of the Nucleolus
The Relationship Between Human Nucleolar Organizer Regions and Nucleoli, Probed by 3D-ImmunoFISH
Analysis of the C. elegans Nucleolus by Immuno-DNA FISH
Correlative Light and Electron Microscopy of Nucleolar Transcription in Saccharomyces cerevisiae
4 High-Throughput Live-Cell Microscopy Analysis of Association Between Chromosome Domains and the Nucleolus in S. cerevisiae
Quantitative Immunofluorescence Analysis of Nucleolus- Associated Chromatin
Visualization of the Nucleolus in Living Cells with Cell- Penetrating Fluorescent Peptides
Analysis of Ribosomal RNA Transcription and Processing
Purification of Crystallization-Grade RNA Polymerase I from S. cerevisiae
Analysis of S. cerevisiae RNA Polymerase I Transcription In Vitro
Preparation of Chromatin Templates to Study RNA Polymerase I Transcription In Vitro
A Nonradioactive Assay to Measure Production and Processing of Ribosomal RNA by 4sU-Tagging
Metabolic Labeling in the Study of Mammalian Ribosomal RNA Synthesis
Quantitative Northern Blot Analysis of Mammalian rRNA Processing
Genomics and Proteomics of the Nucleolus
Complete Sequence Construction of the Highly Repetitive Ribosomal RNA Gene Repeats in Eukaryotes Using Whole Genome Sequence Data
Analysis of rRNA Gene Methylation in Arabidopsis thaliana by CHEF-Conventional 2D Gel Electrophoresis
Fluorescence-Activated Nucleolus Sorting in Arabidopsis
Purification of RNA Polymerase I-Associated Chromatin from Yeast Cells
Bioinformatic Analysis of ChIP-seq Data on the Repetitive Ribosomal RNA Gene
Deep Sequencing Analysis of Nucleolar Small RNAs: RNA Isolation and Library Preparation
Deep Sequencing Analysis of Nucleolar Small RNAs: Bioinformatics
Quantitative Proteomic Analysis of the Human Nucleolus
Analysis of Mass Spectrometry Data for Nucleolar Proteomics Experiments