Fresheney R.I. Culture of Animal Cells: A manual of basic technique and specialized applications

6th edition. — Wiley-Blackwell, 2010. — 796 p.This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs.Biology of Cultured Cells
Laboratory Design, Layout, and Equipment
Equipment and Materials
Aseptic Technique
Safety, Bioethics, and Validation
Culture Vessels and Substrates
Defined Media and Supplements
Serum-Free Media
Preparation and Sterilization
Primary Culture
Subculture and Cell Lines
Cloning and Selection
Cell Separation
Characterization
Differentiation
Transformation and Immortalization
Contamination
Cryopreservation
Quantitation
Cytotoxicity
Specialized Cells
Stem Cells, Germ Cells, and Amniocytes
Culture of Tumor Cells
Three-Dimensional Culture
Scale-up and Automation
Specialized Techniques
Training Programs
Problem Solving