Satir P., Christensen S.T. Cilia: Methods and Protocols

Springer Science+Business Media, New York, 2016. — 275 p. — (Methods in Molecular Biology 1454). — ISBN: 1493937871This volume is the result of an explosion of molecular-based research on Cilia, which began with the discovery of the universality of intraflagellar transport (IFT) and ciliary genomics/proteomics. The chapters in this book cover topics such as: high resolution imaging and functional characterization of sensory and primary cilia in mammalian cells and zebrafish, methods to study ciliary-mediated chemoresponse in Paramecium, and methods to study centrosomes and cilia in C. elegans and Drosophila. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Cilia: Methods and Protocols is broad and covers motile, sensory, and primary cells. It is a valuable resource to anyone interested in entering the field of ciliary biology using model organisms, including flagellate algae, ciliates, planaria, nematodes, insects, zebrafish, and mammalian cells.Methods for Studying Ciliary Import Mechanisms
Targeting of ASH Domain-Containing Proteins to the Centrosome
Morphological and Functional Characterization of the Ciliary Pocket by Electron and Fluorescence Microscopy
Methods to Study Interactions Between Ciliogenesis and Autophagy
Recombinant Reconstitution and Purification of the IFT-B Core Complex from Chlamydomonas reinhardtii
Methods for Studying Movement of Molecules Within Cilia
A FRAP-Based Method for Monitoring Molecular Transport in Ciliary Photoreceptor Cells In Vivo
Kymographic Analysis of Transport in an Individual Neuronal Sensory Cilium in Caenorhabditis elegans
Visualization and Manipulation of Cilia and Intraciliary Calcium in the Zebrafish Left–Right Organizer
Methods for Studying Ciliary-Mediated Chemoresponse in Paramecium
STED and STORM Superresolution Imaging of Primary Cilia
CLEM Methods for Studying Primary Cilia
Methods for Visualization of Neuronal Cilia
Methods to Study Centrosomes and Cilia in Drosophila
Analysis of Axonemal Assembly During Ciliary Regeneration in Chlamydomonas
Planaria as a Model System for the Analysis of Ciliary Assembly and Motility