Michiels J., Fauvart M. Bacterial Persistence. Methods and Protocols

Humana Press, 2016 — 244p. — (Methods in Molecular Biology 1333) — ISBN: 978-1-4939-2854-5 (eBook), 978-1-4939-2853-8 (Hardcover).This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in Escherichia coli cultures, protocols for the determination of the persister subpopulation in Candida albicans, quantitative measurements of Type I and Type II persisters using ScanLag, to in vitro and in vivo models for the study of the intracellular activity of antibiotics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols brings together the most respected researchers in bacterial persistence whose studies will remain vital to understanding this field for many years to come.A Historical Perspective on Bacterial Persistence
Persisters: Methods for Isolation and Identifying Contributing Factors—A Review
A General Method for Measuring Persister Levels in Escherichia coli Cultures
Optimized Method for Measuring Persistence in Escherichia coli with Improved Reproducibility
A Microplate-Based System as In Vitro Model of Biofilm Growth and Quantification
Protocol for Determination of the Persister Subpopulation in Candida Albicans Biofilms
Quantitative Measurements of Type I and Type II Persisters Using ScanLag
Analyzing Persister Physiology with Fluorescence-Activated Cell Sorting
Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array
A Whole-Cell-Based High-Throughput Screening Method to Identify Molecules Targeting Pseudomonas aeruginosa Persister Cells
Functional Analysis of the Role of Toxin–Antitoxin (TA) Loci in Bacterial Persistence
Experimental Evolution of Escherichia coli Persister Levels Using Cyclic Antibiotic Treatments
In Vitro Models for the Study of the Intracellular Activity of Antibiotics
A Murine Model for Escherichia coli Urinary Tract Infection
Analysis of Macrophage-Induced Salmonella Persisters
Population Dynamics Analysis of Ciprofloxacin-Persistent S. Typhimurium Cells in a Mouse Model for Salmonella Diarrhea
Computational Methods to Model Persistence