Leake M.C. Chromosome Architecture. Methods and Protocols

Humana Press, 2016 — 290p. — (Methods in Molecular Biology 1431) — ISBN: 978-1-4939-3631-1 (eBook), 978-1-4939-3629-8 (Hardcover).This volume details a valuable collection of protocols and reviews, such as emerging experimental and theoretical approaches. These approaches have resulted in a substantial improvement in the understanding of chromosome architecture. Chromosome Architecture: Methods and Protocols guides readers through cutting-edge interdisciplinary methods which allow for an understanding of architecture of chromosomes with exceptionally enhanced resolution, both in terms of space and time. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Chromosome Architecture: Methods and Protocols aims to ensure successful results in the further study of this vital field.New Advances in Chromosome Architecture
Single-Molecule Narrow-Field Microscopy of Protein–DNA Binding Dynamics in Glucose Signal Transduction of Live Yeast Cells
Single-Molecule Imaging to Characterize the Transport Mechanism of the Nuclear Pore Complex
Using Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Maintenance of Chromosome (SMC) Complex In Vivo
Imaging DNA Structure by Atomic Force Microscopy
Investigating Bacterial Chromosome Architecture
Transverse Magnetic Tweezers Allowing Coincident Epifluorescence Microscopy on Horizontally Extended DNA
Probing Chromosome Dynamics in Bacillus subtilis
Systems Biology Approaches for Understanding Genome Architecture
In Vivo and In Situ Replication Labeling Methods for Super-resolution Structured Illumination Microscopy of Chromosome Territories and Chromatin Domains
DNA–Protein Interactions Studied Directly Using Single Molecule Fluorescence Imaging of Quantum Dot Tagged Proteins Moving on DNA Tightropes
Escherichia coli Chromosome Copy Number Measurement Using Flow Cytometry Analysis
Bacterial Chromosome Dynamics by Locus Tracking in Fluorescence Microscopy
Biophysical Characterization of Chromatin Remodeling Protein CHD4
Atomistic Molecular Dynamics Simulations of DNA Minicircle Topoisomers: A Practical Guide to Setup, Performance, and Analysis
Super-Resolution Microscopy and Tracking of DNA-Binding Proteins in Bacterial Cells
Studying the Dynamics of Chromatin-Binding Proteins in Mammalian Cells Using Single-Molecule Localisation Microscopy
Intra-Nuclear Single-Particle Tracking (I-SPT) to Reveal the Functional Architecture of Chromosomes
Visualizing Bacillus subtilis During Vegetative Growth and Spore Formation