Isidoro-García M. Molecular Genetics of Asthma

Humana Press, 2016 — 275p. — (Methods in Molecular Biology 1434) — ISBN: 978-1-4939-3652-6 (eBook), 978-1-4939-3650-2 (Hardcover).Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease. Since asthma is a complex disease, this book is designed to provide a review of the most useful techniques with examples of their applications in specific laboratory protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Molecular Genetics of Asthma serves as an ideal guide to researchers investigating this vital field of study.Applications of Molecular Genetics to the Study of Asthma
Assessment and Validation of New Genetic Variants: A Systematic In Silico Approach
Introduction to the Gene Expression Analysis
Real-Time PCR for Gene Expression Quantification in Asthma
Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma
Review of Methods to Study Gene Expression Regulation Applied to Asthma
Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma
SDS-Polyacrylamide Electrophoresis and Western Blotting Applied to the Study of Asthma
Chromatin Immunoprecipitation: Application to the Study of Asthma
Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols
Protocols for Exosome Isolation and RNA Profiling
Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell
Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line
Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line
Review of Mouse Models Applied to the Study of Asthma
General and Specific Mouse Models for Asthma Research
Mouse Models Applied to the Research of Pharmacological Treatments in Asthma
Review on Pharmacogenetics and Pharmacogenomics Applied to the Study of Asthma