Kraayenhof R., Visser A.J.W.G., Gerritsen H.C. (Eds.) Fluorescence Spectroscopy, Imaging and Probes: New Tools in Chemical, Physical and Life Sciences (Springer Series on Fluorescence. Volume 2)

Springer-Verlag, Berlin, Heidelberg, 2002. – 401 p. – ISBN: 978-3-642-62732-3Fluorescence techniques enjoy ever-increasing interest from scientists of a multitude of disciplines: physics, chemistry, biology, geology, pharmacology, toxicology and medicine. Besides widespread fundamental and applied research of fluorescence in university laboratories, one observes a substantially enhanced effort by smaller and larger companies towards the development of new fluorescence-based diagnostic tools. In particular, the application of fluorescence in highthroughput screenings in genomics and proteomics is evidently successful.
This increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. In recent years we observed a vast improvement of microscopic imaging techniques, such as the introduction of confocal scanning microscopy and multi-photon excitation microscopy. Moreover, the design and development of many new molecular probes with higher selectivity for
specific micro-environmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems.
Probably the most significant breakthrough in fluorescence is its use in detection of single molecules and even of their real-time dynamics. Also, probing inside living cells has become a hot topic in the life sciences.
This topic book reflects the updates of scientific progress as presented by invited lecturers and other participants at the t h Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging and Probes (MAF), held in Amsterdam, The Netherlands, 16-19 September 2001.Fluorescence Spectroscopy: New Approaches and Probes
Advanced Luminescent Labels, Probes and Beads and their Application to Luminescence Bioassay and Imaging
Fluorescence Spectral Engineering - Biophysical and Biomedical Applications
Fluorescence Nanometrology in Sol-Gels
Integrated Supramolecular Systems: From Seusors to Switches
Ratiometric Probes: Design and Applications
Binding of Ethidium to Yeast tRNA Phe : A New Perspective on an Old Bromide
Experimental Aspects of DNA Computing by Blocking: Use of Fluorescence Techniques for Detection
Fluorescence Spectroscopy of Single Molecules and Molecular Assemblies
Muitiparametric Detection of Fluorescence Emitted from Individual Muitichromophoric Systems
Fluorescence Intensity Distribution Analysis (FIDA) and related fluorescence fluctuation techniques: theory and practice
Single Molecule Reactions of the Enzyme LDH and of Restriction Endonucleases in the Fluorescence Microscope
Monitoring y-Subunit Movement in Reconstituted Single EFoFl ATP Synthase by Fluorescence Resonance Energy Transfer
Application of Fluorescence in Biological Membrane and Enzyme Studies
Application of the Wavelength-selective Fluorescence Approach to Monitor Membrane Organization and Dynamics
Fluorescence Approaches for the Characterization of the Peripheral Membrane Binding of Proteins Applied for the Blood Coagulation Protein Prothrombin
Assessment of Membrane Fluidity in Individual Yeast Cells by Laurdan Generalised Polarisation and Multi-photon Scanning Fluorescence Microscopy
Formation of Higher Order Signal Transduction Complexes as Seen by Fluorescence Spectroscopy
Mechanisms of the Modulation of Membrane Interfacial Enzyme Catalysis by Non-lamellar Forming Lipids: Comparison with the Behavior of a Fluorescent Probe in Membranes
Emission Spectroscopy of Complex Formation between Escherichia coli Purine Nucleoside Phosphorylase (PNP) and Identified Tautomeric Species of Formycin Inhibitors Resolves Ambiguities Found in Crystallographic Studies
Microscopic Imaging Techniques and their Application for the Study of Living Cells
Fluorescence Lifetime Imaging Implemented with Resonant Galvanometer Scanners
Spectral Imaging of Single CdSe/ZnS Quantum Dots Employing Spectrally- and Time-resolved Confocal Microscopy
Imaging of Oxidative Stress in Plant Cells by Quantitative Fluorescence Microscopy and
The Biomedical Use of Rescaling Procedures in Optical Biopsy and Optical Molecular Imaging
Looking into a Living Cell
Expression of Multicolor Fluorescent Fusion Proteins in Zebrafish Cell Cultures: A Versatile Tool in Cell Biology