Fey P.D. (Ed.) Staphylococcus Epidermidis. Methods and Protocols

Humana Press, 2014 — 208p. — (Methods in Molecular Biology 1106) — ISBN: 978-1-62703-736-5 (eBook), 978-1-62703-735-8 (Hardcover).Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades. The chapters in Staphylococcus Epidermidis: Methods and Protocols are designed to give the new investigator a series of tools so they can ask novel and exciting questions related to the biology of this opportunistic pathogen, as many exciting and unexplored questions such as defining the interaction of S. epidermidis and other normal flora remain to be discovered. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Staphylococcus Epidermidis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.Clinical Characteristics of Infections in Humans Due to Staphylococcus epidermidis
Staphylococcus epidermidis Pathogenesis
Identification of Staphylococcus epidermidis in the Clinical Microbiology Laboratory by Molecular Methods
Pulsed Field Gel Electrophoresis of Staphylococcus epidermidis
Multilocus Sequence Typing of Staphylococcus epidermidis
Growth and Preparation of Staphylococcus epidermidis for NMR Metabolomic Analysis
The Isolation and Analysis of Phenol-Soluble Modulins of Staphylococcus epidermidis
Genetic Manipulation of Staphylococci
Isolation of Chromosomal and Plasmid DNA from Staphylococcus epidermidis
Isolation of Staphylococcus sp. RNA
Use of Electroporation and Conjugative Mobilization for Genetic Manipulation of Staphylococcus epidermidis
Methods to Generate a Sequence-Defined Transposon Mutant Library in Staphylococcus epidermidis Strain 1457
Examination of Staphylococcus epidermidis Biofilms Using Flow-Cell Technology
Rapid Quantitative and Qualitative Analysis of Biofilm Production by Staphylococcus epidermidis Under Static Growth Conditions
Bacteriophage Transduction in Staphylococcus epidermidis
Mouse Model of Post-arthroplasty Staphylococcus epidermidis Joint Infection
A Mouse Model of Staphylococcus Catheter-Associated Biofilm Infection
Generation of a Central Nervous System Catheter-Associated Infection in Mice with Staphylococcus epidermidis
Rat Jugular Catheter Model of Biofilm-Mediated Infection