Spillane C., McKeown P.C. (Eds.) Plant Epigenetics and Epigenomics. Methods and Protocols

Humana Press, 2014 — 245p. — (Methods in Molecular Biology 1112) — ISBN: 978-1-62703-773-0 (eBook), 978-1-62703-772-3 (Hardcover).Many fundamental discoveries concerning epigenetics and the elucidation of mechanisms of epigenetic regulation have developed from studies performed in plants. In Plant Epigenetics and Epigenomics: Methods and Protocols, leading scientists in the epigenetics field describe comprehensive techniques that have been developed to understand the plant epigenetic landscape. These include recently developed methods and techniques for analysis of epigenetically regulated traits, such as flowering time, transposon activation, genomic imprinting and genome dosage effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoidance of known pitfalls.
Authoritative and practical, Plant Epigenetics and Epigenomics: Methods and Protocols seek to aid scientists in the further study of plant epigenetic phenomena using advanced contemporary methods.Landscaping Plant Epigenetics
The Gene Balance Hypothesis: Dosage Effects in Plants
High-Throughput RNA-Seq for Allelic or Locus-Specific Expression Analysis in Arabidopsis-Related Species, Hybrids, and Allotetraploids
Inference of Allele-Specific Expression from RNA-seq Data
Screening for Imprinted Genes Using High-Resolution Melting Analysis of PCR Amplicons
Analysis of Genomic Imprinting by Quantitative Allele-Specific Expression by Pyrosequencing
Endosperm-Specific Chromatin Profiling by Fluorescence-Activated Nuclei Sorting and Chip-on-Chip
Imaging Sexual Reproduction in Arabidopsis Using Fluorescent Markers
Genome-Wide Analysis of DNA Methylation in Arabidopsis Using MeDIP-Chip
Methylation-Sensitive Amplified Polymorphism (MSAP) Marker to Investigate Drought-Stress Response in Montepulciano and Sangiovese Grape Cultivars
Detecting Histone Modifications in Plants
Quantitatively Profiling Genome-Wide Patterns of Histone Modifications in Arabidopsis thaliana Using ChIP-seq
Analysis of Retrotransposon Activity in Plants
Detecting Epigenetic Effects of Transposable Elements in Plants
Detection and Investigation of Transitive Gene Silencing in Plants