Storici F. (Ed.) Gene Correction. Methods and Protocols

Humana Press, 2014 — 463p. — (Methods in Molecular Biology 1114) — ISBN: 978-1-62703-761-7 (eBook), 978-1-62703-760-0 (Hardcover).Gene correction is a technology that gives us the tools for both repairing and mutating DNA, for discovering gene functions and for engineering new genetic variants. Gene Correction: Methods and Protocols provides a user friendly, detailed and up-to-date collection of strategies and methodologies utilized for generating specific sequence changes in the DNA of cells in the laboratory, while also tackling the major problems that the field of gene correction faces. This volume brings together many experts in the field of gene correction to disclose a wide and varied array of specific gene correction protocols for engineering mutations in DNA, for delivering correcting DNA to target cells, and for improving the accuracy and safety of the gene correction process. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Gene Correction: Methods and Protocols seeks to serve scientists of all backgrounds interested in the area of gene targeting/recombination/therapy.RecTE_Psy-Mediated Recombineering in Pseudomonas syringae
Genome Manipulations with Bacterial Recombineering and Site-Specific Integration in Drosophila
Multiple Genetic Manipulations of DT40 Cell Line
Gene Targeting of Human Pluripotent Stem Cells by Homologous Recombination
Methods for the Assessment of ssODN-Mediated Gene Correction Frequencies in Muscle Cells
Small Fragment Homologous Replacement (SFHR): Sequence-Specific Modification of Genomic DNA in Eukaryotic Cells by Small DNA Fragments
Preparation and Application of Triple Helix Forming Oligonucleotides and Single Strand Oligonucleotide Donors for Gene Correction
Triplex-Mediated Genome Targeting and Editing
Targeting piggyBac Transposon Integrations in the Human Genome
Gene Targeting in Human-Induced Pluripotent Stem Cells with Adenoviral Vectors
Enhanced Gene Targeting of Adult and Pluripotent Stem Cells Using Evolved Adeno-associated Virus
Lentiviral Vectors Encoding Zinc-Finger Nucleases Specific for the Model Target Locus HPRT1
Designing and Testing the Activities of TAL Effector Nucleases
A Bacterial One-Hybrid System to Isolate Homing Endonuclease Variants with Altered DNA Target Specificities
Design and Analysis of Site-Specific Single-Strand Nicking Endonucleases for Gene Correction
CRISPR-Cas-Mediated Targeted Genome Editing in Human Cells
RNA-Guided Genome Editing of Mammalian Cells
Nuclease-Mediated Double-Strand Break (DSB) Enhancement of Small Fragment Homologous Recombination (SFHR) Gene Modification in Human-Induced Pluripotent Stem Cells (hiPSCs)
AAV-Mediated Gene Editing via Double-Strand Break Repair
Genetic Modification Stimulated by the Induction of a Site-Specific Break Distant from the Locus of Correction in Haploid and Diploid Yeast Saccharomyces cerevisiae