Alexandrov K., Johnston W.A. (Eds.) Cell-Free Protein Synthesis. Methods and Protocols

Humana Press, 2014. — 313 p. — (Methods in Molecular Biology 1118). — ISBN: 978-1-62703-782-2; 978-1-62703-781-5.Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the conversion of DNA encoded genetic information into protein-mediated biochemical activities. Cell-Free Protein Synthesis: Methods and Protocols brings together the key opinion leaders of cell-free technology development and provides case studies and detailed protocols for the application of cell-free methodology. Chapters cover the main directions in the development of cell-free technologies including several recently developed cell-free systems, as well as a number of applications of cell-free systems ranging from discovery of biofuel enzymes to in vitro assembly of viruses. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Cell-Free Protein Synthesis: Methods and Protocols seeks to serve a wide variety of scientists with its well-honed methodologies.Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolae
Bioinformatics Analysis and Optimization of Cell-Free Protein Synthesis
A Cell-Free Expression Screen to Identify Fusion Tags for Improved Protein Expression
One-Pot, Microscale Cell-Free Enzyme Expression and Screening
Cell-Free Translation of Biofuel Enzymes
Cloning-Independent Expression and Screening of Enzymes Using Cell-Free Protein Synthesis Systems
High-Level Cell-Free Production of Membrane Proteins with Nanodiscs
Cell-Free Protein-Based Enzyme Discovery and Protein–Ligand Interaction Study
Human Cell Extract-Derived Cell-Free Systems for Virus Synthesis
Cell-Free Protein Synthesis in Microfluidic 96-Well Plates
Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis
Site-Specific Incorporation of Unnatural Amino Acids into Proteins by Cell-Free Protein Synthesis
In Vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free Lysate
An Optimized Yeast Cell-Free Lysate System for In Vitro Translation of Human Virus mRNA
In Vitro Translation-Based Protein Kinase Substrate Identification
Preparation of Protein Arrays Using Cell-Free Protein Expression
Posttranscriptional Control of Protein Synthesis in Drosophila S2 Cell-Free System
Cell-Free Membrane Protein Expression
The PURE System for Protein Production
A Cell-Free Protein Synthesis System from Insect Cells