Yurochko M.D., Miller W.E. (Eds.) Human Cytomegaloviruses. Methods and Protocols

Humana Press, 2014 — 368p. — (Methods in Molecular Biology 1119) — ISBN: 978-1-62703-788-4 (eBook), 978-1-62703-787-7 (Hardcover).With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed. Human Cytomegaloviruses: Methods and Protocols is designed to be an inclusive document covering all of the techniques and approaches necessary to understand and study the pathobiology of human cytomegalovirus. Topics covered include the history of human cytomegaloviruses, techniques to culture and grow the virus in model cell types, the use of primary cells for the study of human cytomegalovirus pathogenesis, modern molecular techniques for assessing the biological consequences of viral infection, animal models for study of cytomegalovirus replication and numerous other topics of current interest. Written in the successful Methods in Molecular Biology series format, each chapter includes an introduction, list of necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Human Cytomegaloviruses: Methods and Protocols serves as a tool for basic scientists as well as clinical scientists with an interest in the basic fundamental aspects of viral gene expression and specific aspects of viral pathogenesis.History of the Molecular Biology of Cytomegaloviruses
Overview of Human Cytomegalovirus Pathogenesis
Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies
Use of Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegalovirus Infection
Use of Recombinant Approaches to Construct Human Cytomegalovirus Mutants
The Use of Primary Human Cells (Fibroblasts, Monocytes, and Others) to Assess Human Cytomegalovirus Function
Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation
Analysis of Cytomegalovirus Binding/Entry-Mediated Events
Use of 5-Ethynyl-2′-Deoxyuridine Labelling and Flow Cytometry to Study Cell Cycle-Dependent Regulation of Human Cytomegalovirus Gene Expression
Methods for Studying the Function of Cytomegalovirus GPCRs
Methods for the Detection of Cytomegalovirus in Glioblastoma Cells and Tissues
Methods to Study the Nucleocytoplasmic Transport of Macromolecules with Respect to Their Impact on the Regulation of Human Cytomegalovirus Gene Expression
Fluorescence-Based Laser Capture Microscopy Technology Facilitates Identification of Critical In Vivo Cytomegalovirus Transcriptional Programs
Techniques for Characterizing Cytomegalovirus-Encoded miRNAs
What We Have Learned from Animal Models of HCMV
Rodent Models of Congenital Cytomegalovirus Infection
Recent Approaches and Strategies in the Generation of Antihuman Cytomegalovirus Vaccines
Approaches for the Generation of New Anti-cytomegalovirus Agents: Identification of Protein–Protein Interaction Inhibitors and Compounds Against the HCMV IE2 Protein