Fontecilla-Camps J.C., Nicolet Y. (Eds.) Metalloproteins. Methods and Protocols

Humana Press, 2014 — 299p. — (Methods in Molecular Biology 1122) — ISBN: 978-1-62703-794-5 (eBook), 978-1-62703-793-8 (Hardcover).Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mössbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.Advances in Research on Metalloproteins
Purification of O₂-Sensitive Metalloproteins
Expression and Purification of NifB Proteins from Aerobic and Anaerobic Sources
Techniques for the Production, Isolation, and Analysis of Iron–Sulfur Proteins
Cell-Free Synthesis of the H-Cluster: A Model for the In Vitro Assembly of Metalloprotein Metal Centers
Electrochemistry of Metalloproteins: Protein Film Electrochemistry for the Study of E. coli [NiFe]-Hydrogenase-1
FTIR Spectroscopy of Metalloproteins
Characterizing Millisecond Intermediates in Hemoproteins Using Rapid-Freeze-Quench Resonance Raman Spectroscopy
A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and Model Compounds
Study of Metalloproteins Using Continuous Wave Electron Paramagnetic Resonance (EPR)
Mössbauer Spectroscopy
X-Ray Absorption Spectroscopy of Metalloproteins
X-Ray Crystallographic Studies of Metalloproteins
Quantum Mechanical Methods for the Investigation of Metalloproteins and Related Bioinorganic Compounds
Density Functional Theory–Broken Symmetry (DFT–BS) Methodology Applied to Electronic and Magnetic Properties of Bioinorganic Prosthetic Groups