Quinn M.T., DeLeo F.R. (Eds.) Neutrophil Methods and Protocols

Humana Press, 2014 — 2nd Ed. — 551p. — (Methods in Molecular Biology 1124) — ISBN: 978-1-62703-845-4 (eBook), 978-1-62703-844-7 (Hardcover).Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. Neutrophil Methods and Protocols, Second Edition provides a concise set of protocols written by leading researchers in the field for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step diagnostic assays of common neutrophil disorders. Topics covered include an overview of neutrophils and their role in host defense and inflammation; methods most commonly used for isolating neutrophils from humans and other animal species; procedures for subcellular fractionation of human neutrophils, analysis of in vivo transmigrated neutrophils, generation of mature neutrophils from induced pluripotent stem cells and analysis of neutrophil gene expression; methods for investigating priming, oxidant production, phagocytosis, bactericidal activity and extracellular trap formation and protocols for investigating neutrophil adhesion, chemotaxis and outside-in signaling via integrins. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Neutrophil Methods and Protocols, Second Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.The Role of Neutrophils in the Immune System: An Overview
Isolation of Human Neutrophils from Venous Blood
Neutrophil Isolation from Nonhuman Species
Collection of In Vivo Transmigrated Neutrophils from Human Skin
Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers
Rho Family and Rap GTPase Activation Assays
Measurement of Phospholipid Metabolism in Intact Neutrophils
Optical Methods for the Measurement and Manipulation of Cytosolic Calcium Signals in Neutrophils
Analysis of Electrophysiological Properties and Responses of Neutrophils
Assessment of Neutrophil Apoptosis
Microinjection Methods for Neutrophils
Generation of Functionally Mature Neutrophils from Induced Pluripotent Stem Cells
Neutrophil Migration Through Extracellular Matrix
Spinning Disk Confocal Imaging of Neutrophil Migration in Zebrafish
Detection of Bidirectional Signaling During Integrin Activation and Neutrophil Adhesion
Immunofluorescence and Confocal Microscopy of Neutrophils
Expression of Genetically Encoded Fluorescent Probes to Monitor Phospholipid Dynamics in Live Neutrophils
Quantitative Assessment of Neutrophil Phagocytosis Using Flow Cytometry
Analysis of Neutrophil Bactericidal Activity
Induction and Quantification of Neutrophil Extracellular Traps