Rorbach J., Bobrowicz A.J. (Eds.) Polyadenylation. Methods and Protocols

Humana Press, 2014 — 377p. — (Methods in Molecular Biology 1125) — ISBN: 978-1-62703-971-0 (eBook), 978-1-62703-970-3 (Hardcover).In Polyadenylation: Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study polyadenylation. Focusing on recent advances in the fast-moving polyadenylation filed, that has recently been recognized as a key contributor to the complexity of mammalian gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.3′ End Formation and Regulation of Eukaryotic mRNAs
Retracted: Poly(A) Polymerase-Based Poly(A) Length Assay
Using Klenow-Mediated Extension to Measure Poly(A)-Tail Length and Position in the Transcriptome
Detection of Uridylated mRNAs
Cytoplasmic Polyadenylation Assays
In Vitro Analysis of Transcriptional Activators and Polyadenylation
Fluorescence Detection of Polyadenylation Reaction Through the Coordination of Adenosine₂–Coralyne–Adenosine₂
Small Molecule Recognition of Poly(A)
The Stem-Loop Luciferase Assay for Polyadenylation (SLAP) Method for Determining CstF-64-Dependent Polyadenylation Activity
Accurate Mapping of Cleavage and Polyadenylation Sites by 3′ Region Extraction and Deep Sequencing
Polyadenylation Site Prediction Using PolyA-iEP Method
Detecting Alternative Polyadenylation from Microarray Data
Quantitative Polyadenylation Site Mapping with Single-Molecule Direct RNA Sequencing
RHAPA: A New Method to Quantify Alternative Polyadenylation
Multiplex Analysis of PolyA-Linked Sequences (MAPS): An RNA-Seq Strategy to Profile Poly(A+) RNA
Global and Quantitative Profiling of Polyadenylated RNAs Using PAS-seq
High-Throughput Sequencing of RNA Isolated by Cross-Linking and Immunoprecipitation (HITS-CLIP) to Determine Sites of Binding of CstF-64 on Nascent RNAs
Polyadenylation in Bacteria and Organelles
In Vivo Analysis of Polyadenylation in Prokaryotes
Polyadenylation of RNA in E. coli: RNA Polymerase-Associated (rA)_n-Synthetic Activities