Labrou N.E. (Ed.) Protein Downstream Processing. Design, Development and Application of High and Low-Resolution Methods

Humana Press, 2014 — 555p. — (Methods in Molecular Biology 1129) — ISBN: 978-1-62703-977-2 (eBook), 978-1-62703-976-5 (Hardcover).Proteins are the most diverse group of biologically important substances. With the recent technological advances in the genomics area and the efforts in proteomics research, the rate of discovery for new proteins with unknown structure and function has increased. These proteins generated from genomic approaches present enormous opportunities for research and industrial application. Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods is a compilation of chapters within the exciting area of protein purification designed to give the laboratory worker the information needed to design and implement a successful purification strategy. It presents reliable and robust protocols in a concise form, emphasizing the critical aspects on practical problems and questions encountered at the lab bench. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods will be an ideal source of scientific information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, and biotechnology and other related areas in both academia and industry.Protein Purification: An Overview
Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoE
High-Throughput Process Development: I. Process Chromatography
High-Throughput Process Development: II. Membrane Chromatography
Media Selection in Ion-Exchange Chromatography in a Single Microplate
High-Throughput Screening of Dye-Ligands for Chromatography
Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips
Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline
Aqueous Two-Phase System Strategies for the Recovery of Proteins from Plants
Aqueous Two-Phase-Assisted Precipitation of Proteins: A Platform for Isolation of Process-Related Impurities from Therapeutic Proteins
Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro (Affinity Ligand)-Facilitated Three Phase Partitioning (MLFTPP)
Co-expression and Co-purification of Antigen–Antibody Complexes in Bacterial Cytoplasm and Periplasm
Immunoglobulin Purification by Caprylic Acid
Affinity Tags in Protein Purification and Peptide Enrichment: An Overview
Expression and Purification of Large Active GST Fusion Enzymes
Synthetic Ligand Affinity Chromatography Purification of Human Serum Albumin and Related Fusion Proteins
Z_basic: A Purification Tag for Selective Ion-Exchange Recovery
An Orthogonal Fusion Tag for Efficient Protein Purification
Phage Display of Engineered Binding Proteins
Biomimetic Affinity Ligands for Protein Purification